RESUMO
We tested the effects of vitamin A supplementation on the antibody titre of 40 healthy volunteers (age range: 10-35 years), who had received a complete course of antirabies vaccine (5 injections over 30 days). After determining the baseline serum vitamin A status of 80 volunteers, 20 pairs were matched for serum vitamin A level, body mass index, age, sex and socioeconomic status. One person from each pair was randomly assigned to an experimental or control group. The experimental group received vitamin A and antirabies vaccine. Controls received only the vaccine. The experimental group had significantly greater (2.1 times) serum antirabies titre than controls. This finding is an important step towards improving the economy of dosage of antirabies vaccines.
Assuntos
Formação de Anticorpos/efeitos dos fármacos , Vacina Antirrábica/imunologia , Vitamina A/uso terapêutico , Adolescente , Adulto , Anticorpos Antivirais/sangue , Anticorpos Antivirais/efeitos dos fármacos , Formação de Anticorpos/imunologia , Índice de Massa Corporal , Criança , Cromatografia Líquida de Alta Pressão , Terapia Combinada , Suplementos Nutricionais , Sinergismo Farmacológico , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imunocompetência/efeitos dos fármacos , Imunocompetência/imunologia , Masculino , Paquistão , Vacina Antirrábica/administração & dosagem , Vírus da Raiva/imunologia , Fatores Socioeconômicos , Inquéritos e Questionários , Resultado do Tratamento , Vacinação/métodos , Vitamina A/sangue , Vitamina A/imunologia , Deficiência de Vitamina A/sangue , Deficiência de Vitamina A/tratamento farmacológico , Deficiência de Vitamina A/imunologiaRESUMO
We tested the effects of vitamin A supplementation on the antibody titre of 40 healthy volunteers [age range: 10-35 years], who had received a complete course of antirabies vaccine [5 injections over 30 days]. After determining the baseline serum vitamin A status of 80 volunteers, 20 pairs were matched for serum vitamin A level, body mass index, age, sex and socioeconomic status. One person from each pair was randomly assigned to an experimental or control group. The experimental group received vitamin A and antirabies vaccine. Controls received only the vaccine. The experimental group had significantly greater [2.1 times] serum antirabies titre than controls. This finding is an important step towards improving the economy of dosage of antirabies vaccines
Assuntos
Anticorpos Antivirais , Formação de Anticorpos , Índice de Massa Corporal , Cromatografia Líquida de Alta Pressão , Terapia Combinada , Sinergismo Farmacológico , Ensaio de Imunoadsorção Enzimática , Imunocompetência , Vacina Antirrábica , Vírus da Raiva , Vacinação , Deficiência de Vitamina A , Vitamina ARESUMO
A rapid, sensitive and specific high-performance liquid chromatographic method was developed for the determination of serum levels of retinol in humans. A direct serum injection technique after deproteinisation was used to avoid lengthy pretreatment steps which can result in degradation of retinol during analysis. The column used was CLC-ODS, the mobile phase was acetonitrile-water and detection wavelength was 328 nm. Deterioration in column performance was not observed even after injection of 300 samples. The lower detection limit was 10 micrograms/l. On analyzing a serum pool six times, a C.V. of 0.7% was obtained. The method is quantitative, reproducible, rapid and highly accurate for routine analysis.
